In conclusion we suggest that long-term FRHT intake affects exploration and anxiety-related behaviour of the rats as well as exerts biochemical outcomes in the brain that support the neuroprotective impact of rooibos tea.Īmino acids Aspalathus linearis Brain Hole-board Rat Rooibos herbal tea.Ĭopyright © 2021 Elsevier B.V. Excitatory amino acids (aspartate and glutamate) content was decreased in the striatum of animals drinking the infusions whereas taurine level was increased both in the striatum and hippocampus. Analisa penelitian ini menggunakan statistik dengan uji anova satu arah. Longer time of the testing-box central zone occupation indicated to reduction in anxiety-related behaviour. Hole Board Test dilakukan dengan cara mengamati dan menghitung jumlah mencit mencelupkan kepala yang sudah diberi sediaan uji dan ditempatkan pada kandang dengan partisi kayu yang sudah dilubangi. Their exploration measured by head-dipping and rearing was enhanced. The rats drinking rooibos tea presented increased motor activity defined as time spent on moving in the HBT. that the olfactory hole-board test is a valuable tool for measuring avoidance and preference responses to biologically relevant odors. Brain amino acids concentration was analysed in the striatum, hippocampus and prefrontal cortex by HPLC-ECD. Their behaviour was evaluated in the hole-board test (HBT). FRHTs of different leaf to water ratios (1:100, 2:100 and 4:100), analysed by chromatographic methods as regards their flavonoid characteristics, were given to rats as only liquid for 3 months. We hypothesized that it might affect brain amino acid content and thus induce behaviour and neuroprotection. The present research was taken to extend the knowledge about the feasible behavioural and neurochemical implications of sustained oral FRHT consumption. It was already shown that "fermented" rooibos herbal tea (FRHT), which is rich in phenolic compounds, improves the cognitive performance of rats in the water maze and impacts dopaminergic striatal transmission.
linearis is connected with the ability of reducing glycaemia, inflammation as well as oxidative stress. This correlation turned out to be less successful in performing the modified hole board test.Rooibos tea, brewed using Aspalathus linearis leaves, is a popular South African herbal infusion, but its everyday intake is not fully described in terms of the neuropsychopharmacological outcomes. In BL6 mice, cognition was correlated to general exploration. Principal component analysis indicated a close correlation between anxiety-related behaviour and cognitive performance in DBA mice, being associated with a highly successful cognitive performance. During cognitive testing, DBA mice habituated faster and performed better than BL6 mice. Principal component analysis on the phenotyping data showed that anxiety-related behaviour was described by identical parameters and was not correlated to locomotion in the two strains.
We could show that naive BL6 and DBA mice distinctly differed in terms of anxiety- related behaviour. Because it has never been evaluated whether cognitive performance and emotional behaviour are interrelated in these strains, we analysed various behavioural dimensions and cognitive functions in parallel using the modified hole board test.
We investigated the interaction between behavioural dimensions and cognitive performance in the inbred mouse strains C57BL/6 and DBA/2, which have previously been found to differ in cognitive performance and emotionality.